Journal: Small Science

Article Title: The Importance of Particle Shape: Effect of Nonspherical Particles and Their Stabilized Pickering Emulsions on Immunization Efficacy

doi: 10.1002/smsc.202400527

Figure Lengend Snippet: Characterization of PNT‐stabilized Pickering emulsions (PNTEs). a) Schematic diagram of preparation of PNTEs. b) CLSM images of the cross section and surface of PNTEs, squalene was labeled by Nile red (yellow) and PNTs were labeled by Cy5 (red). c) Cryo‐SEM image of PNTEs.

Article Snippet: Cy5 mono‐reactive N‐hydroxysuccinimide esters was purchased from Fanbo Biochemicals (China).

Techniques: Labeling

Journal: Small Science

Article Title: The Importance of Particle Shape: Effect of Nonspherical Particles and Their Stabilized Pickering Emulsions on Immunization Efficacy

doi: 10.1002/smsc.202400527

Figure Lengend Snippet: Interaction between vaccine adjuvants and APCs. CLSM images of nonspherical particles: a) PMFs, b) PMTs, and c) PNTs in contact with J774a.1 macrophage. Particles were labeled with Cy5 (green), F‐actin was labeled with phalloidin‐FITC (red), and nuclei were labeled with DAPI (blue) (scale bar = 5 μm). d) Schematic diagram of interaction between adjuvant and APCs. e) Antigen uptake rates of DC2.4 with different antigen‐loaded particles or emulsions ( n = 6) and its flow cytometry representative diagram. f) QCM‐D analyzed the deformability of particles. g) QCM‐D analyzed the deformability of emulsions. CLSM images of J774a.1 macrophage uptake of PNTEs h) before and i) after, F‐actin was labeled with phalloidin‐FITC (green), PNTs were labeled with Cy5 (purple), and squalene was labeled with Nile red (cyan) (scale bar = 1 μm). j) Representative flow cytometry images of recruited APCs on day 3 by vaccine adjuvants.

Article Snippet: Cy5 mono‐reactive N‐hydroxysuccinimide esters was purchased from Fanbo Biochemicals (China).

Techniques: Labeling, Adjuvant, Flow Cytometry, QCM-D

Journal: Advanced Science

Article Title: Remediation of Metal Oxide Nanotoxicity with a Functional Amyloid

doi: 10.1002/advs.202310314

Figure Lengend Snippet: Rescue of HUVEC early/late cell apoptosis and cell necrosis induced by CuO and ZnO nanoparticles with bLg amyloid. A) Flow cytometry was used to classify necrotic and apoptotic HUVECs upon 24 h of exposure to CuO or ZnO nanoparticles (100, 150 µ m ) in the presence and absence of the bLg amyloid (5 mg mL −1 ). Apoptotic cells were labeled with Annexin V‐FITC, and necrotic cells were labeled with propidium iodide (Q3‐1: necrosis. Q3‐2: late apoptosis. Q3‐3: normal cells. Q3‐4: early apoptosis). B) Statistical evaluation of normal, apoptotic, and necrotic (%) cells in the control and treated cell groups presented in panel (A). Data were analyzed via two‐way ANOVA followed by Tukey's post‐hoc test for multiple comparisons (n = 3). C) Adhesion rate of the bLg amyloid on the surface of HUVECs in the presence and absence of endocytosis inhibitors. HUVECs were treated with MβCD (5 m m for 2 h) and MDC (10 µ m for 1 h) before the bLg treatment. HUVECs were exposed to the bLg amyloid labeled with Cy5 (Cy5‐bLg) for 4 h, and Cy5 positive cells were detected by flow cytometry. D) Statistical analysis of Cy5 positive cells derived from the assay presented in panel (C). Data were analyzed via one‐way ANOVA followed by Tukey's post‐hoc test for multiple comparisons (n = 3). E) Confocal fluorescence images depicting the localization of the Cy5‐bLg amyloid (2.5 mg mL −1 ) in non‐/pre‐treated (MβCD/MDC) HUVECs upon exposure for 4 h. Channels: Nuclei (blue), Cy5‐bLg amyloid (purple), F‐actin (green). Scale bars: 20 µm, n = 3. All the data are depicted as mean ± SD. Significance between the treated and control groups is denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001. Similarly, # p < 0.05, ## p < 0.01, and ### p < 0.001 represented the significant differences between two treated groups.

Article Snippet: For the synthesis of cyanine5‐labeled bLg (Cy5‐bLg) amyloid, 10 mg of the mono‐reactive succinimidyl dye ester of Cy5 (NHS‐Cy5, Pierce, USA) was completely dissolved in 1 mL of dimethyl sulfoxide.

Techniques: Flow Cytometry, Labeling, Control, Derivative Assay, Fluorescence